Journal: PLoS ONE
Article Title: The Nucleosome (Histone-DNA Complex) Is the TLR9-Specific Immunostimulatory Component of Plasmodium falciparum That Activates DCs
doi: 10.1371/journal.pone.0020398
Figure Lengend Snippet: FL-DCs prepared from the bone marrow cells of WT mice were plated in 24-well plates and stimulated with merozoites (MZs), parasite nuclear material or polynucleosomes. DCs stimulated with MZs or CpG ODN was used as controls. The upregulated surface expression of costimulatory molecules, CD40, CD80 and CD86, were analyzed by flow cytometry. The percentages of DCs that are positive to each costimulatory molecule are indicated. Data are representatives of two independent experiments.
Article Snippet: Fluorescein isothiocyante (FITC)-conjugated antibodies against mouse CD3e (clone 145-2C11), CD11c (clone N418) and pan NK cells (clone DX5), phycoerythrin (PE)-conjugated anti-mouse NK1.1 antibody (clone PK136), PE-Cy5-conjugated antibodies against mouse CD80 (clone 16-10A1), hamster IgG (clone eBio299Arm) isotype control, CD86 (clone GL1) and rat IgG2aκ isotype control, and allophycocyanin (APC)-conjugated antibodies against mouse CD40 (clone 1C10) and rat IgG2aκ isotype control were from eBioscience (San Diego, CA).
Techniques: Expressing, Flow Cytometry